Part:BBa_M36148:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_M36148

Insertion into Saccharomyces cerevisiae through either pD1201 or pD1231, found on DNA 2.0. This part has successfully transformed into S. cerevisiae using pD1201.

Stanford Location Plasmid Name = AlgA DNA 2.0 Gene # = pD1201 Organism = Saccharomyces cerevisiae Device Type = Actuator Barcode # of Glycerol Stock = 0133011781, 0133011367, 0133027021, 0133024492 Box Label = BioE44 F15 Box 2

User Reviews

UNIQc2377406b3509657-partinfo-00000000-QINU Chloe Rickards, Review #1

A team of three individuals used this part in a classroom lab space, in BIOE 44 at Stanford University. We failed to detect the production of alginate A with our part. However, more tests need to be done in order to definitively conclude this part as non-functional. We inserted this gene into a plasmid with a galactose inducible promotor, but failed to introduce galactose into our media until later in the experiment. As a result, we could not detect AlgA through Western blotting. We expect better results either by introducing galactose into the system earlier, or by using a strong constitutive promoter. Additionally, we experienced difficulties measuring fructose-6-phosphate consumption using our BioVision PicoProbe fructose-6-phosphate assay. Future experimenters should either choose a different method through which to measure fructose-6-phosphate consumption, or find a way to measure mannose-6-phosphate directly. UNIQc2377406b3509657-partinfo-00000001-QINU